1OT9

CRYOTRAPPED STATE IN WILD TYPE PHOTOACTIVE YELLOW PROTEIN, INDUCED WITH CONTINUOUS ILLUMINATION AT 110K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	110
Detector technology	CCD
Collection date	2001-02-26
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	66.206, 66.206, 40.514
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.000
R-factor	0.134
Rwork	0.132
R-free	0.16100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	2.290
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.040
High resolution limit [Å]	1.000	1.000
Rmerge	0.057	0.568
Number of reflections	54493
<I/σ(I)>	30.8	7.2
Completeness [%]	98.6	95.4
Redundancy	15.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		ammonium sulphate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 110K
1	VAPOR DIFFUSION, HANGING DROP	7		ammonium sulphate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 110K