1OT9
CRYOTRAPPED STATE IN WILD TYPE PHOTOACTIVE YELLOW PROTEIN, INDUCED WITH CONTINUOUS ILLUMINATION AT 110K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2001-02-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 66.206, 66.206, 40.514 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.000 |
R-factor | 0.134 |
Rwork | 0.132 |
R-free | 0.16100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 2.290 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.040 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.057 | 0.568 |
Number of reflections | 54493 | |
<I/σ(I)> | 30.8 | 7.2 |
Completeness [%] | 98.6 | 95.4 |
Redundancy | 15.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | ammonium sulphate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 110K | |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | ammonium sulphate, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 110K |