1OSY
Crystal structure of FIP-Fve fungal immunomodulatory protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-07 |
| Detector | PHILLIPS |
| Wavelength(s) | 0.9202 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 97.118, 97.118, 61.413 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.020 - 1.700 |
| R-factor | 0.18358 |
| Rwork | 0.182 |
| R-free | 0.21000 * |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.466 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.045 | 0.187 |
| Total number of observations | 464964 * | |
| Number of reflections | 32880 | 3249 * |
| <I/σ(I)> | 28.1 | 16.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 21 * | PEG 400, ammonium sulfate, Tris-base, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 2.5 (%(w/v)) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 3 | 1 | reservoir | Tris base | 0.1 (M) | pH8.5 |
