1OSU
STRUCTURE OF THE RNA HEXAMER, R(UUCGCG), WITH A 5'-UU-OVERHANG EXHIBITING HOOGSTEEN-LIKE TRANS U-U BASE PAIRS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 30.600, 34.000, 29.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.400 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 29.000 * |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.400 |
| Rmerge | 0.024 * |
| Number of reflections | 2991 |
| Completeness [%] | 86.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.3 * | 4 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | ISOPROPANOL | ||
| 3 | 1 | 1 | MG2+ | ||
| 4 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 40 (%) | |
| 2 | 1 | drop | nonamer | ||
| 3 | 1 | drop | lithium cacodylate | 20 (mM) | |
| 4 | 1 | drop | 6-10 (mM) | or Ir(NH3)Cl3 |
