1OSM
OSMOPORIN (OMPK36) FROM KLEBSIELLA PNEUMONIAE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 277 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.800, 76.800, 223.400 |
| Unit cell angles | 90.00, 113.30, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.200 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2omf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.105 | 0.320 |
| Number of reflections | 47412 | |
| <I/σ(I)> | 3.7 | 2.1 |
| Completeness [%] | 96.1 | 97.2 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 9.8 | pH 9.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | C8HESO | 0.6 (%) | |
| 2 | 1 | 1 | octyl-POE | 0.1 (%) | |
| 3 | 1 | 1 | 0.5 (M) | ||
| 4 | 1 | 1 | Tris-HCl | 0.05 (M) | |
| 5 | 1 | 1 | PEG2000 | 15 (%) |
