1OSD
crystal structure of Oxidized MerP from Ralstonia metallidurans CH34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.810, 52.612, 60.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 2.000 |
| R-factor | 0.1997 |
| Rwork | 0.192 |
| R-free | 0.26754 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afi |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.306 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.335 |
| Number of reflections | 9437 | |
| <I/σ(I)> | 10.7 | 2.1 |
| Completeness [%] | 97.0 | 97 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEGMME5000, Sodium acetate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
