1OSC
Crystal structure of rat CUTA1 at 2.15 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9322 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.387, 88.287, 125.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.1954 |
| Rwork | 0.189 |
| R-free | 0.26000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1naq |
| RMSD bond length | 0.030 * |
| RMSD bond angle | 2.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.80) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.073 | 0.470 |
| Total number of observations | 300781 * | |
| Number of reflections | 46060 * | 6646 * |
| <I/σ(I)> | 5.3 | 1.8 |
| Completeness [%] | 100.0 * | 100 * |
| Redundancy | 6.5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 20 * | Na Acetate, CuSO4, CaCl2, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 3 | 1 | reservoir | MPD | 30 (%) | |
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | 20 (mM) |
