1OSB
Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free structure.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-20 |
Detector | MACSCIENCE |
Spacegroup name | P 65 |
Unit cell lengths | 147.780, 147.780, 78.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.650 |
R-factor | 0.1962 |
Rwork | 0.195 |
R-free | 0.24140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omh |
RMSD bond length | 0.012 |
RMSD bond angle | 1.484 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.098 | 0.291 |
Number of reflections | 28480 | |
<I/σ(I)> | 7 | 2.5 |
Completeness [%] | 99.5 | 97.1 |
Redundancy | 4.4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 4 * | 25% PEGMM 2000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 296KK |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SODIUM ACETATE | ||
2 | 1 | 1 | AMMONIUM SULPHATE | ||
3 | 1 | 2 | SODIUM ACETATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | PEG2000 MME | 25 (%(w/v)) | |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |