1OS4
Dehydrated T6 human insulin at 295 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.900, 49.440, 49.260 |
| Unit cell angles | 115.12, 104.01, 102.69 |
Refinement procedure
| Resolution | 41.700 * - 2.250 |
| Rwork | 0.243 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ins |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.200 * |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.640 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.070 | 0.289 |
| Total number of observations | 15512 * | |
| Number of reflections | 10252 | |
| <I/σ(I)> | 7.3 | 1.6 |
| Completeness [%] | 82.7 * | 85.9 |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.3 | 295 | hydrochloric acid, zinc acetate, sodium citrate, acetone, pH 6.3, SLOW COOLING, temperature 295.K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 5 (mg/ml) | |
| 2 | 1 | 1 | 0.01 (M) | ||
| 3 | 1 | 1 | zinc acetate | 0.007 (M) | |
| 4 | 1 | 1 | sodium citrate | 0.05 (M) | |
| 5 | 1 | 1 | acetone | 17 (%) | pH6.3 |
