1OS4
Dehydrated T6 human insulin at 295 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2002-06-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 33.900, 49.440, 49.260 |
Unit cell angles | 115.12, 104.01, 102.69 |
Refinement procedure
Resolution | 41.700 * - 2.250 |
Rwork | 0.243 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ins |
RMSD bond length | 0.007 |
RMSD bond angle | 22.200 * |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.640 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.070 | 0.289 |
Total number of observations | 15512 * | |
Number of reflections | 10252 | |
<I/σ(I)> | 7.3 | 1.6 |
Completeness [%] | 82.7 * | 85.9 |
Redundancy | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.3 | 295 | hydrochloric acid, zinc acetate, sodium citrate, acetone, pH 6.3, SLOW COOLING, temperature 295.K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | insulin | 5 (mg/ml) | |
2 | 1 | 1 | 0.01 (M) | ||
3 | 1 | 1 | zinc acetate | 0.007 (M) | |
4 | 1 | 1 | sodium citrate | 0.05 (M) | |
5 | 1 | 1 | acetone | 17 (%) | pH6.3 |