1OS3
Dehydrated T6 human insulin at 100 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-06-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 81.510, 81.510, 33.760 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.420 - 1.950 |
Rwork | 0.216 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ins |
RMSD bond length | 0.006 |
RMSD bond angle | 20.200 * |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.420 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.045 | 0.175 |
Total number of observations | 22524 * | |
Number of reflections | 6070 | |
<I/σ(I)> | 15.9 | 3.67 |
Completeness [%] | 99.6 * | 100 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.3 | 295 | hydrochloric acid, zinc acetate, sodium citrate, acetone, pH 6.3, SLOW COOLING, temperature 295.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | insulin | 5 (mg/ml) | |
2 | 1 | 1 | 0.01 (M) | ||
3 | 1 | 1 | zinc acetate | 0.007 (M) | |
4 | 1 | 1 | sodium citrate | 0.05 (M) | |
5 | 1 | 1 | acetone | 17 (%) | pH6.3 |