Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.782, 93.782, 132.249 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.000 * - 2.000* |
| Rwork | 0.147 |
| R-free | 0.17300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lnf |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.022 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (- TRUNCATE) |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 * | 2.300 |
| High resolution limit [Å] | 2.000 * | 2.000 * |
| Rmerge | 0.076 | 0.125 * |
| Number of reflections | 23742 * | |
| <I/σ(I)> | 9 | 4.1 |
| Completeness [%] | 91.6 * | 85.5 * |
| Redundancy | 5.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 278 | DMSO, CaCl2, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 120 (mg/ml) | |
| 2 | 1 | drop | inhibitor | 20 (mM) | |
| 3 | 1 | reservoir | Tris acetate | 50 (mM) | pH8.0 |
| 4 | 1 | reservoir | calcium acetate | 0.5 (M) | |
| 5 | 1 | reservoir | dithiothreitol | 4 (mM) | |
| 6 | 1 | reservoir | sodium azide | 1 (mM) | |
| 7 | 1 | reservoir | DMSO | 45 (%(v/v)) |
