1ORC

CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-09-26
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	34.770, 39.170, 48.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.540
R-factor	0.178 *
Rwork	0.178
RMSD bond length	0.015
RMSD bond angle	2.300
Data reduction software	SDMS
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.540 *
Rmerge	0.029
Total number of observations	45435 *
Number of reflections	9834
Completeness [%]	95.8
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		20 (mM)
2	1	drop	EDTA	0.1 (mM)
3	1	drop	protein	16 (mg/ml)
4	1	reservoir	sodium formate	4.2-4.8 (M)