1OR7

Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-26
Detector	MARRESEARCH
Wavelength(s)	.9792
Spacegroup name	C 1 2 1
Unit cell lengths	140.049, 56.901, 104.981
Unit cell angles	90.00, 130.51, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.19879
Rwork	0.197
R-free	0.23200 *
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.480 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.069 *	0.381 *
Total number of observations	181227 *
Number of reflections	82833
<I/σ(I)>	16.4	3.6
Completeness [%]	98.3	92.8 *
Redundancy	2.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	4 *	sodium formate, MES buffer, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	sodium formate	1.6 (M)
3	1	reservoir	MES	0.1 (M)	pH6.0