1OR3

APOLIPOPROTEIN E3 (APOE3), TRIGONAL TRUNCATION MUTANT 165

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	125
Detector technology	AREA DETECTOR
Collection date	1997-05-15
Detector	ADSC
Spacegroup name	P 31 2 1
Unit cell lengths	47.370, 47.370, 104.540
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.730
R-factor	0.229 *
Rwork	0.229
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	UCSD-system
Data scaling software	UCSD-system
Phasing software	SOLVE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.860
High resolution limit [Å]	1.730	1.730
Rmerge	0.068	0.225
Total number of observations	48854 *
Number of reflections	16782
<I/σ(I)>	27.4	3.7
Completeness [%]	97.2	89.9 *
Redundancy	2.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6		RT, 50MM NA-CACODYLATE, PH 5.6, 10-20% PEG 400, 1% 2-ME. NOTE: W/O 2-ME OR HIGHER PEG CONCENTRATIONS, OTHER CRYSTAL FORMS APPEARS (SEE PDB ENTRIES 1BZ4, 1OR2).

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	2	50MM NA-Cacodylate, pH 5.6
2	1	2	10-20% PEG 400
3	1	2	1% 2-ME

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	20-25 (%)
2	1	reservoir	sodium cacodylate	50 (mM)
3	1	drop		20 (mM)
4	1	drop	protein	5 (mg/ml)