1OR2
APOLIPOPROTEIN E3 (APOE3) TRUNCATION MUTANT 165
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 125 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-04-15 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.680, 55.590, 63.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.252 * |
| Rwork | 0.267 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bz4 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.500 * |
| Data reduction software | UCSD-system (DATA REDUCTION PACKAGE) |
| Data scaling software | UCSD-system |
| Phasing software | EPMR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.680 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.069 | 0.245 |
| Total number of observations | 39148 * | |
| Number of reflections | 5913 | |
| <I/σ(I)> | 29.7 | 4.4 |
| Completeness [%] | 93.8 | 89.5 * |
| Redundancy | 6.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 50MM NA-CACODYLATE, PH 5.6, 10-20% PEG 400 AT ROOM TEMPERATURE. NO 2-ME ADDED. NOTE: WITH 2-ME OR LOWER PEG CONCENTRATIONS, OTHER CRYSTAL FORMS APPEARS (SEE PDB ENTRIES 1BZ4, 1OR3), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 20-25 (%) | |
| 2 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | protein | 5 (mg/ml) |
