1OQW
Full-Length PAK Pilin from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.015, 44.422, 73.782 |
| Unit cell angles | 90.00, 116.82, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DZO.pdb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.329 * |
| Total number of observations | 324152 * | |
| Number of reflections | 29308 * | |
| <I/σ(I)> | 14.8 | 2.4 |
| Completeness [%] | 97.2 | 83.6 |
| Redundancy | 3.24 | 2.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.6 | 293 | 15% MPD, 35% PEG 4000, 100 mM sodium citrate, 2.5 mM MnCl2, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 15 (%) | |
| 3 | 1 | reservoir | PEG4000 | 35 (%) | |
| 4 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 5 | 1 | reservoir | 2.5 (mM) |
