1OQQ
Crystal structure of C73S/C85S mutant of putidaredoxin, a [2Fe-2S] ferredoxin from Pseudomonas putida, at 1.47A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2002-12-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 50.212, 75.845, 107.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.080 - 1.470 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.21800 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.080 | 1.500 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.049 * | 0.548 |
Number of reflections | 34883 | |
<I/σ(I)> | 9.8 | 1.78 |
Completeness [%] | 98.2 | 98.8 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 298 | ammonium sulfate, sodium citrate, sodium/potassium phosphate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | drop | Bis-Tris propane/acetate | 100 (mM) | pH7.4 |
3 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
4 | 1 | reservoir | sodium citrate | 50 (mM) | pH5.9 |
5 | 1 | reservoir | sodium potassium phospahte | 300 (mM) |