1OQM
A 1:1 complex between alpha-lactalbumin and beta1,4-galactosyltransferase in the presence of UDP-N-acetyl-galactosamine
Replaces: 1L7WExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.471, 95.677, 100.581 |
| Unit cell angles | 90.00, 101.40, 90.00 |
Refinement procedure
| Resolution | 19.560 - 2.100 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.27000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 * |
| RMSD bond angle | 0.010 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 * | |
| Total number of observations | 120498 * | |
| Number of reflections | 62285 * | |
| <I/σ(I)> | 13.7 | 2.01 |
| Completeness [%] | 90.0 * | 76 |
| Redundancy | 2.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * | 298 | Sodium citrate, PEG4000, pH 5.5, LIQUID DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Gal-T1 | 20 (mg/ml) | |
| 2 | 1 | drop | alpha-lactalbumin | 10 (mg/ml) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 5 | 1 | reservoir | PEG4000 | 12.5 (%) |
