1OQJ
Crystal structure of the SAND domain from glucocorticoid modulatory element binding protein-1 (GMEB1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-13 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9185 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.946, 49.077, 88.903 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 * - 1.550 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.21000 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 23.000 * |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.450 | 1.600 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.047 * | 0.108 * |
Number of reflections | 26235 | 2085 * |
<I/σ(I)> | 29.22 | 7.15 |
Completeness [%] | 98.8 | 88.6 |
Redundancy | 6.87 | 2.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 100 mM Hepes, 1.4 M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
2 | 1 | reservoir | sodium cirtate | 1.4 (M) | |
3 | 1 | drop | protein | 15 (mg/ml) |