1OQE

Crystal structure of sTALL-1 with BAFF-R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 63 2 2
Unit cell lengths	233.261, 233.261, 211.286
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.264
Rwork	0.244
R-free	0.26500 *
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	27.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500 *	2.450
Rmerge	0.135 *	0.600 *
Total number of observations	1415809 *
Number of reflections	121940
<I/σ(I)>	11
Completeness [%]	99.3	90
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9		Liu, Y., (2002) Cell, 108, 383. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	beta-mercaptoethanol	5 (mM)
3	1	reservoir	Bicine	50 (mM)	pH9.0
4	1	reservoir		150 (mM)
5	1	reservoir	dioxane	35-38 (%)