1OQD
Crystal structure of sTALL-1 and BCMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 232.854, 232.854, 212.477 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.870 - 2.600 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.23500 * |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.129 * | 0.550 * |
| Total number of observations | 1056950 * | |
| Number of reflections | 97672 * | |
| <I/σ(I)> | 10 | 58 |
| Completeness [%] | 94.0 * | 46.2 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9 | 277 | Liu, Y., (2002) Cell, 108, 383. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 3 | 1 | reservoir | Bicine | 50 (mM) | pH9.0 |
| 4 | 1 | reservoir | 150 (mM) | ||
| 5 | 1 | reservoir | dioxane | 35-38 (%) |
