1OQC

The crystal structure of augmenter of liver regeneration: a mammalian FAD dependent sulfhydryl oxidase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	120
Detector technology	CCD
Collection date	1998-09-21
Detector	CUSTOM-MADE
Wavelength(s)	0.95, 0.979, 0.978
Spacegroup name	P 21 21 2
Unit cell lengths	106.987, 123.130, 37.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.650 - 1.800
R-factor	0.202
Rwork	0.202
R-free	0.24000
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.650	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	41788
<I/σ(I)>		2.88
Completeness [%]	76.5	40.5
Redundancy	4.8	4.02

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	300	PEG 4000, magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	6.5	300	PEG 4000, magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	6.5	300	PEG 4000, magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K