1OQ9
The Crystal Structure of the Complex between Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean) and Acetate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2001-06-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.987 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 94.110, 94.110, 81.783 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.400 |
R-factor | 0.21812 |
Rwork | 0.216 |
R-free | 0.26400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.020 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 253.000 * | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.043 * | 0.283 * |
Number of reflections | 16245 | |
<I/σ(I)> | 13.5 | 2.8 |
Completeness [%] | 99.4 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 293 | 12-15% Peg4000, 80mM Cacodylate, 200mM Magnesium Acetate, 75mM Ammonium Sulphate, 0.2% Octyl glucoside, 20% Glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium azide | 70 (mM) | |
2 | 1 | reservoir | cacodylate | 0.08 (M) | pH5.4 |
3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 75 (mM) | |
5 | 1 | reservoir | octyl-glucoside | 0.2 (%) | |
6 | 1 | reservoir | PEG4000 | 12-15 (%) |