1OQ9
The Crystal Structure of the Complex between Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean) and Acetate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-06-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 94.110, 94.110, 81.783 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.21812 |
| Rwork | 0.216 |
| R-free | 0.26400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 253.000 * | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.043 * | 0.283 * |
| Number of reflections | 16245 | |
| <I/σ(I)> | 13.5 | 2.8 |
| Completeness [%] | 99.4 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 293 | 12-15% Peg4000, 80mM Cacodylate, 200mM Magnesium Acetate, 75mM Ammonium Sulphate, 0.2% Octyl glucoside, 20% Glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium azide | 70 (mM) | |
| 2 | 1 | reservoir | cacodylate | 0.08 (M) | pH5.4 |
| 3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 75 (mM) | |
| 5 | 1 | reservoir | octyl-glucoside | 0.2 (%) | |
| 6 | 1 | reservoir | PEG4000 | 12-15 (%) |
