1OQ7
The crystal structure of the iron free (Apo-)form of Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-10-09 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 188.033, 188.033, 82.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.960 - 3.200 |
| R-factor | 0.22935 |
| Rwork | 0.229 |
| R-free | 0.25700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.520 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.065 * | 0.196 * |
| Number of reflections | 49227 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 92.7 * | 65.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 277 | 8-10% Peg6000, 60mM Strontium Chloride, 0.1M ADA buffer, 15% Glycerol, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium azide | 70 (mM) | |
| 2 | 1 | reservoir | cacodylate | 0.08 (M) | pH5.4 |
| 3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 75 (mM) | |
| 5 | 1 | reservoir | octyl-glucoside | 0.2 (%) | |
| 6 | 1 | reservoir | PEG4000 | 12-15 (%) |
