1OQ4
The Crystal Structure of the Complex between Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean) and Azide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.986 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.774, 145.210, 192.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.920 - 2.400 |
R-factor | 0.22888 |
Rwork | 0.229 |
R-free | 0.24100 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1afr |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.057 * | 0.299 * |
Number of reflections | 90115 | |
<I/σ(I)> | 14.8 | |
Completeness [%] | 99.3 * | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 293 | 70mM Sodium Azide, 12-15% Peg4000, 80mM Cacodylate, 200 mM Magnesium Acetate, 75mM Ammonium Sulphate, 0.2% Octyl glucoside and water exchanged for 20% MPD for freezing, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium azide | 70 (mM) | |
2 | 1 | reservoir | cacodylate | 0.08 (M) | pH5.4 |
3 | 1 | reservoir | magnesium acetate | 200 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 75 (mM) | |
5 | 1 | reservoir | octyl-glucoside | 0.2 (%) | |
6 | 1 | reservoir | PEG4000 | 12-15 (%) |