1OQ1
Crystal Structure of Protein of Unknown Function with Galectin-like Fold from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-06 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.839, 44.616, 139.391 |
| Unit cell angles | 90.00, 100.34, 90.00 |
Refinement procedure
| Resolution | 46.210 - 1.700 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.20800 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.210 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.066 | 0.230 |
| Number of reflections | 88537 | |
| <I/σ(I)> | 8.6 | 4.2 |
| Completeness [%] | 89.0 | 51.4 |
| Redundancy | 5.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Ammonium Acetate, BisTris, PEG10000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
