1OPX
Crystal structure of the traffic ATPase (HP0525) of the Helicobacter pylori type IV secretion system bound by sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-08 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 111.280, 111.280, 230.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 2.800 |
| R-factor | 0.252 |
| Rwork | 0.250 |
| R-free | 0.32400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.360 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.890 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.115 * | 0.156 * |
| Total number of observations | 80168 * | |
| Number of reflections | 18699 | |
| Completeness [%] | 87.0 | 94 * |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 | 298 | ammonium sulfate, peg 400, tris-HCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 2 | 1 | reservoir | PEG400 | 2 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.8 |
