1OPR
THE CRYSTAL STRUCTURE OF THE OROTATE PHOSPHORIBOSYLTRANSFERASE COMPLEXED WITH OROTATE AND ALPHA-D-5-PHOSPHORIBOSYL-1-PYROPHOSPHATE
Experimental procedure
Spacegroup name | P 41 21 2 |
Unit cell lengths | 47.300, 47.300, 216.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.187 * |
Rwork | 0.187 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.500 |
Phasing software | X-PLOR |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | |
High resolution limit [Å] | 2.030 * | 2.030 * |
Rmerge | 0.080 * | |
Total number of observations | 38356 * | |
Number of reflections | 12880 | 1182 * |
Completeness [%] | 77.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30-40 (mg/ml) | |
2 | 1 | drop | orotidine monophosphate | 10 (mM) | |
3 | 1 | drop | ammonium sulfate | 65 (%sat) | |
4 | 1 | drop | NaHepes | 70 (mM) | |
5 | 1 | drop | 0.05 (mM) | ||
6 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
7 | 1 | reservoir | PEG4000 | 30-40 (%(w/v)) | |
8 | 1 | reservoir | NaHepes | 70 (mM) |