1OOA
CRYSTAL STRUCTURE OF NF-kB(p50)2 COMPLEXED TO A HIGH-AFFINITY RNA APTAMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.753, 151.059, 95.628 |
| Unit cell angles | 90.00, 105.95, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.450 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nfk |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.250 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.059 | 0.496 * |
| Total number of observations | 519385 * | |
| Number of reflections | 37890 | |
| <I/σ(I)> | 15.7 | 3 |
| Completeness [%] | 99.0 * | 99 |
| Redundancy | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6 | 291 | PEG 8000, ethylene glycol, ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | ethylene glycol | ||
| 3 | 1 | 1 | ammonium sulfate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | PEG8000 | 14 (%) | ||
| 4 | 1 | 1 | sodium citrate | 100 (mM) | pH6.0 |
| 5 | 1 | 1 | beta-octylglucoside | 0.5 (%) |
