1OO2
Crystal structure of transthyretin from Sparus aurata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.452, 65.622, 70.834 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 48.000 * - 1.560 |
| R-factor | 0.1973 |
| Rwork | 0.197 |
| R-free | 0.22300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f41 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.020 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 * | 1.640 |
| High resolution limit [Å] | 1.560 * | 1.560 |
| Rmerge | 0.055 | 0.146 * |
| Total number of observations | 239286 * | |
| Number of reflections | 60660 | |
| <I/σ(I)> | 7 | 7 |
| Completeness [%] | 97.2 | 89.9 |
| Redundancy | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 20 * | PEG 400, cadmio chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 3 | 1 | reservoir | PEG400 | 15 (%(w/v)) | pH4.6 |
