1ONN
IspC apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-05-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.548 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.396, 53.419, 107.490 |
Unit cell angles | 90.00, 92.85, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.25 |
Rwork | 0.244 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k5h |
RMSD bond length | 0.011 |
RMSD bond angle | 1.410 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.062 | 0.405 |
Number of reflections | 31997 | |
Completeness [%] | 98.2 | 98.4 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 * | 21 * | PEG 4000, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 100 (mM) | pH8.0 |
10 | 1 | reservoir | EDTA | 10 (mM) | |
11 | 1 | reservoir | dithiothreitol | 12 (mM) | pH5.8 |
2 | 1 | drop | dithiothreitol | 2 (mM) | |
3 | 1 | drop | 0.02 (%(m/v)) | ||
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | reservoir | PEG4000 | 8 (%(w/v)) | |
6 | 1 | reservoir | sodium acetate | 100 (mM) | |
7 | 1 | reservoir | HEPES | 110 (mM) | |
8 | 1 | reservoir | glycine | 100 (mM) | |
9 | 1 | reservoir | guanidinium chloride | 100 (mM) |