1ONK
Mistletoe lectin I from viscum album
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-05-17 |
Detector | MARRESEARCH |
Wavelength(s) | 0.84530 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 107.285, 107.285, 311.773 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
R-factor | 0.1629 |
Rwork | 0.160 |
R-free | 0.22330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2mll |
RMSD bond length | 0.006 |
RMSD bond angle | 0.027 |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.052 | 0.396 |
Number of reflections | 60505 | |
<I/σ(I)> | 16.05 | |
Completeness [%] | 96.9 | 93 |
Redundancy | 6.4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 2.5 | 293 | 40% saturated ammonium sulphate, 30% glycerol in 0.1M, pH 2.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |