1ONJ

Crystal structure of Atratoxin-b from Chinese cobra venom of Naja atra

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRL BEAMLINE U7B
Synchrotron site	NSRL
Beamline	U7B
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2002-06-25
Detector	MARRESEARCH
Wavelength(s)	1.1516
Spacegroup name	P 41 21 2
Unit cell lengths	49.110, 49.110, 44.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.570 * - 1.560 *
Rwork	0.171
R-free	0.19250
Structure solution method	MOLECULAR REPLACEMENT
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.570	1.640
High resolution limit [Å]	1.560 *	1.560
Rmerge	0.082 *	0.390 *
Total number of observations	87491 *
Number of reflections	8305 *
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	293	ammonium sulfate, Tris-HCl, pH 8.5, EVAPORATION, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
3	1	reservoir	ammonium sulfate	2.0 (M)