1ONE

YEAST ENOLASE COMPLEXED WITH AN EQUILIBRIUM MIXTURE OF 2'-PHOSPHOGLYCEATE AND PHOSPHOENOLPYRUVATE

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	121.900, 73.200, 93.900
Unit cell angles	90.00, 93.30, 90.00

Refinement procedure

Resolution	60.000 - 1.800
Rwork	0.177
RMSD bond length	0.018
RMSD bond angle	2.100
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.033 *	0.132 *
Total number of observations	113340 *
Number of reflections	71494
Completeness [%]	92.0 *	81 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8 *		macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enolase	15 (mg/ml)
2	1	1	PEG8000	15 (%)
3	1	1		0.25 (M)
4	1	1	HEPPS/KOH	50 (mM)
5	1	1	P-enolpyruvate	2 (mM)
6	1	1		2 (mM)