1ONC

THE REFINED 1.7 ANGSTROMS X-RAY CRYSTALLOGRAPHIC STRUCTURE OF P-30, AN AMPHIBIAN RIBONUCLEASE WITH ANTI-TUMOR ACTIVITY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	40.530, 69.640, 32.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 1.700
R-factor	0.178
Rwork	0.178
RMSD bond length	0.016
RMSD bond angle	0.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.050 *
Total number of observations	43858 *
Number of reflections	9495 *
Completeness [%]	89.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	ammonium sulfate	25 (%sat)
3	1	drop	acetate	5 (mM)
4	1	reservoir	ammonium sulfate	50 (%sat)
5	1	reservoir	acetate	10 (mM)