1ON2
Bacillus subtilis Manganese Transport Regulator (MntR), D8M Mutant, Bound to Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 100 |
Spacegroup name | P 32 |
Unit cell lengths | 52.500, 52.500, 83.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.600 - 1.610 |
Rwork | 0.220 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.060 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 1.670 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.043 | 0.266 |
Total number of observations | 101987 * | |
Number of reflections | 30208 | |
<I/σ(I)> | 10.4 | 2.4 |
Completeness [%] | 91.6 | 62.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 293 | 30% PEG 400, 0.15 M Lithium sulfate, pH 8.5, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
3 | 1 | reservoir | PEG400 | 20-30 (%(v/v)) | |
4 | 1 | reservoir | 0.15 (M) | ||
5 | 1 | reservoir | 2 (mM) |