1ON0
Crystal Structure of Putative Acetyltransferase (YycN) from Bacillus subtilis, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET SR144
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.982 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.891, 101.594, 62.120 |
| Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
| Resolution | 29.680 - 2.200 |
| R-factor | 0.259 |
| Rwork | 0.225 |
| R-free | 0.28900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.680 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.071 | 0.354 |
| Number of reflections | 69770 | |
| <I/σ(I)> | 16.64 | 3.74 |
| Completeness [%] | 91.5 | 99.1 |
| Redundancy | 3.26 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 5mM Tris, 20% PEG 8k, 0.2 M Ammonium Sulfate, 50 mM Sodium Chloride, and 5 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
