1OMS
Structure determination by MAD: E.coli Trigger Factor binding at the ribosomal exit tunnel.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9797, 0.9404 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 71.644, 71.644, 375.409 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.900 - 2.300 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.059 | 0.330 |
| Number of reflections | 31810 * | |
| <I/σ(I)> | 33.3 | 6.6 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.2 * | 4.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 6 * | PEG 2000 MME, PEG 400, ammonium sulfate, sodium acetate, tris, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.5 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 MME | 30 (%) | |
| 3 | 1 | reservoir | sodium acetate | 85 (mM) | |
| 4 | 1 | reservoir | Tris-base | 100 (mM) | |
| 5 | 1 | reservoir | glycerol | 15 (%) |
