1OMH
Conjugative Relaxase TrwC in complex with OriT Dna. Metal-free structure.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2002-03-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795, 0.9798, 0.9796 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 90.830, 90.830, 203.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.950 |
R-factor | 0.2487 |
Rwork | 0.246 |
R-free | 0.27970 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.692 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 2.050 | 1.950 |
Rmerge | 0.146 | |
Number of reflections | 64999 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 4 * | 25% PEGMM 2000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 4 * | 25% PEGMM 2000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2(SO4) | ||
2 | 1 | 1 | (Na)(C2H3O2) | ||
3 | 1 | 1 | PEG | ||
4 | 1 | 2 | (Na)(C2H3O2) |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | PEG2000 MME | 25 (%(w/v)) | |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |