1OM8

CRYSTAL STRUCTURE OF A COLD ADAPTED ALKALINE PROTEASE FROM PSEUDOMONAS TAC II 18, CO-CRYSTALLYZED WITH 10 mM EDTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-04
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	H 3
Unit cell lengths	181.700, 181.700, 37.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.194
Rwork	0.177
R-free	0.20800
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.590
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.100	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.091 *	0.195 *
Total number of observations	115986 *
Number of reflections	30897
Completeness [%]	99.8	99.2
Redundancy	3.8	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	Villeret, V., (1997) Protein Sci., 6, 2462. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.6 (M)
2	1	reservoir	HEPES	0.1 (M)	pH7.0
3	1	drop	protein	15 (mg/ml)