1OM4

STRUCTURE OF RAT NEURONAL NOS HEME DOMAIN WITH L-ARGININE BOUND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-01-07
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 21 21 21
Unit cell lengths	51.890, 110.190, 164.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.980 - 1.750
R-factor	0.22
Rwork	0.220
R-free	0.24500
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmerge		0.598
Number of reflections	95384
<I/σ(I)>	13.3	2.8
Completeness [%]	98.4	98
Redundancy	5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	280	PEG4000, MES, ammonium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 280K