1OM1

Crystal structure of maize CK2 alpha in complex with IQA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-07
Detector	MARRESEARCH
Wavelength(s)	1.2
Spacegroup name	C 1 2 1
Unit cell lengths	141.524, 60.276, 44.546
Unit cell angles	90.00, 102.63, 90.00

Refinement procedure

Resolution	32.800 - 1.680
Rwork	0.206
R-free	0.22600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.500 *
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.500	1.770
High resolution limit [Å]	1.680	1.680
Rmerge	0.069 *	0.069 *
Number of reflections	41163 *	5404 *
Completeness [%]	97.8 *	97.8 *
Redundancy	2.9 *	2.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	283 *	PEG 4000, SODIUM ACETATE, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	inhibitor solution	100 (mM)	in 100%(v/v) DMSO
3	1	reservoir	PEG4000	20 (%(w/v))
4	1	reservoir	sodium acetate	0.2 (M)
5	1	reservoir	Tris	0.1 (M)	pH8.