1OLZ

The ligand-binding face of the semaphorins revealed by the high resolution crystal structure of SEMA4D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-20
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	73.320, 76.760, 89.410
Unit cell angles	77.41, 73.35, 63.57

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.206
Rwork	0.206
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	28.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.099	0.801
Total number of observations	375205 *
Number of reflections	108424
<I/σ(I)>	17.6	1.8
Completeness [%]	97.2	90.9
Redundancy	3.5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *		0.2 M AMMONIUM FLORIDE, 20% PEG 3350, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)	pH8.0
3	1	drop		0.1 (M)