1OLT
Coproporphyrinogen III oxidase (HemN) from Escherichia coli is a Radical SAM enzyme.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 114.036, 114.036, 76.459 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 2.070 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.18700 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.066 | 0.182 |
| Number of reflections | 34220 * | |
| <I/σ(I)> | 30.6 | 10.2 |
| Completeness [%] | 99.1 | 96.8 |
| Redundancy | 9.4 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | HANGING DROP: 5 MICROL PROTEIN + 5 MICROL RESEVOIR, PROTEIN: 10 MG/ML IN 10 MM HEPES PH 7.5, 3 MM DTT, 150 MM NACL, 0.01% (V/V) C8E4 RESEVOIR: 22% (W/V) PEG10000, 100 MM HEPES PH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | SAM | 1 (mM) | |
| 3 | 1 | reservoir | PEG10000 | 22 (%) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
