1OLO
Hexameric Replicative DNA Helicase RepA from Plasmid RSF1010 - Cubic Crystal Structure
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 190.293, 190.293, 190.293 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.550 |
| Rwork | 0.176 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| Starting model (for MR) | 1g8y |
| RMSD bond length | 0.021 * |
| RMSD bond angle | 1.838 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 | 2.580 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.106 | 0.620 * |
| Total number of observations | 305969 * | |
| Number of reflections | 40046 | |
| <I/σ(I)> | 13.6 | 3.3 |
| Completeness [%] | 99.1 | 89.7 |
| Redundancy | 7.64 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 291 | SITTING DROP VAPOR DIFFUSION AT 291K. PROTEIN STOCK: 26MG/ML REPA, 10MM TRIS-HCL PH8.0, 150MM NACL, 0.1MM EDTA. PRECIPITANT: 100MM TRIS-HCL PH 7.5, 26% PEG400, 100MM MGSO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 26 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |
| 6 | 1 | reservoir | PEG400 | 26 (%) | |
| 7 | 1 | reservoir | 100 (mM) |
