1OKS

Crystal structure of the measles virus phosphoprotein XD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-13
Detector	ADSC CCD
Spacegroup name	P 32 2 1
Unit cell lengths	50.109, 50.109, 47.127
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 1.800
R-factor	0.2
Rwork	0.198
R-free	0.24600 *
Structure solution method	SIRAS
RMSD bond length	0.020 *
RMSD bond angle	1.630 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.052	0.472
Total number of observations	44883 *
Number of reflections	6611
<I/σ(I)>	6.5	1.6
Completeness [%]	99.6	100
Redundancy	6.8	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	20 *	0.1 M CHES PH 8.5, 1.25 M NA CITRATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	reservoir	CHES	0.1 (M)	pH8.5
3	1	reservoir	sodium citrate	1.25 (M)