1OKO
Crystal structure of Pseudomonas Aeruginosa Lectin 1 complexed with galactose at 1.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.057, 53.275, 160.675 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.600 * - 1.600 |
| R-factor | 0.1557 |
| Rwork | 0.154 |
| R-free | 0.18700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MONOMER B FROM PREVIOULSY RESOLVED 1OKP |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 1.570 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.076 | 0.410 |
| Total number of observations | 241596 * | |
| Number of reflections | 53762 | |
| <I/σ(I)> | 6.6701 | 1.42 |
| Completeness [%] | 94.9 | 79.1 |
| Redundancy | 4.5 * | 4.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 * | HANGING DROP: PROTEIN SOLUTION: PA1L 5 MG/ML, D-GAL 0.025 MG/ML, CACL2 & MGCL2 2MM RESERVOIR SOLUTION: (NH4)2SO4 1.5 M, PH 4.7, 5% MPD, 2% GLYCEROL 2 UL + 2 UL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.5 (M) | pH4.7 |
| 3 | 1 | reservoir | MPD | 5 (%) | |
| 4 | 1 | reservoir | glycerol | 2 (%) |
