1OKJ
crystal structure of the essential E. coli YeaZ protein by MAD method using the gadolinium complex "DOTMA"
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Collection date | 2003-06-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.340, 97.600, 141.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.280 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.045 | 0.136 |
| Number of reflections | 48235 | |
| <I/σ(I)> | 11 | 1.9 |
| Completeness [%] | 98.3 | 98.5 |
| Redundancy | 5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.7 | PEG 8000, NA ACETATE 0.1M PH 4.7, NACL 0.2M, GLYCEROL 10% |
