1OK9
Decay accelerating factor (CD55): The structure of an intact human complement regulator.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.130, 54.600, 62.770 |
| Unit cell angles | 87.19, 85.61, 65.74 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-free | 0.27400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojv |
| RMSD bond length | 0.004 |
| RMSD bond angle | 21.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | TNT (5F) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.053 | 0.114 |
| Number of reflections | 10372 | |
| <I/σ(I)> | 9.1 | 5.8 |
| Completeness [%] | 93.0 | 92.7 |
| Redundancy | 3.8 | 3.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.6 | 4 * | 0.2 M AMMONIUM SULPHATE, 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6, 16% GLYCEROL THEN SOAKED IN 2 MM K2PTCL4 FOR 6 DAYS |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 2 | 1 | reservoir | MPEG5000 | 20 (%(w/v)) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 4 | 1 | reservoir | glycerol | 10 (%(w/v)) |
