1OK8
Crystal structure of the dengue 2 virus envelope glycoprotein in the postfusion conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 76.151, 76.151, 130.775 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.26710 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1OAM |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.410 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.398 |
| Number of reflections | 27450 * | |
| <I/σ(I)> | 25 | 2 |
| Completeness [%] | 95.0 | 75 |
| Redundancy | 7 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 20 * | 20-30% PEG 400, 0.08 M NACL, 0.1 M MOPS PH 7-8. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | TEA | 8 (nM) | pH7. |
| 2 | 1 | drop | 80 (mM) | ||
| 3 | 1 | drop | UDM | 3 (mM) | |
| 4 | 1 | drop | protein | 15 (mg/ml) | |
| 5 | 1 | reservoir | PEG400 | 20-30 (%) | |
| 6 | 1 | reservoir | MOPS | 0.1 (M) | pH7-8 or pH8-9 |
| 7 | 1 | reservoir | 80 (mM) |
