1OK6
Orthorhombic crystal form of an Archaeal fructose 1,6-bisphosphate aldolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Collection date | 2002-02-15 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.900, 176.500, 185.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.390 * |
R-factor | 0.233 |
Rwork | 0.232 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ojx |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.470 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.121 | 0.372 |
Number of reflections | 106518 | |
<I/σ(I)> | 13.3 | 2.7 |
Completeness [%] | 93.6 | 69 |
Redundancy | 5.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * | 9% (W/V) PEG 4000, 0.1 M NA ACETATE PH 5.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3 (mg/ml) | |
2 | 1 | drop | HEPES-NaOH | 10 (mM) | pH7.5 |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | PEG4000 | 9 (%(w/v)) | |
6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |